Culturing:
The majority of modern culture techniques still take a CFU-f
approach, where raw unpurified bone marrow or ficoll-purified
bone marrow monocytes are plated directly into cell culture plates
or flasks. Mesenchymal stem cells, but not red blood cells or
haematopoetic progenitors, are adherent to tissue culture plastic
within 24 to 48 hours. However, at least one publication has
identified a population of non-adherent MSCs that are not obtained
by the direct-plating technique.
Other flow cytometry-based methods allow the sorting of bone
marrow cells for specific surface markers, such as STRO-1.
STRO-1+ cells are generally more homogenous, and have higher
rates of adherence and higher rates of proliferation, but the exact
differences between STRO-1+ cells and MSCs are not clear.
The majority of modern culture techniques still take a CFU-f
approach, where raw unpurified bone marrow or ficoll-purified
bone marrow monocytes are plated directly into cell culture plates
or flasks. Mesenchymal stem cells, but not red blood cells or
haematopoetic progenitors, are adherent to tissue culture plastic
within 24 to 48 hours. However, at least one publication has
identified a population of non-adherent MSCs that are not obtained
by the direct-plating technique.
Other flow cytometry-based methods allow the sorting of bone
marrow cells for specific surface markers, such as STRO-1.
STRO-1+ cells are generally more homogenous, and have higher
rates of adherence and higher rates of proliferation, but the exact
differences between STRO-1+ cells and MSCs are not clear.
- Wan C, He Q, McCaigue M, Marsh D, Li G (2006). "Nonadherent cell population of human marrow
culture is a complementary source of mesenchymal stem cells (MSCs)". Journal of Orthopaedic
Research 24 (1): 21–8. doi:10.1002/jor.20023. PMID 16419965.
- Gronthos S, Graves SE, Ohta S, Simmons PJ (1994). "The STRO-1+ fraction of adult human
bone marrow contains the osteogenic precursors". Blood 84 (12): 4164–73. PMID 7994030.
- Oyajobi BO, Lomri A, Hott M, Marie PJ (1999). "Isolation and characterization of human
clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO-1
monoclonal antibody". Journal of Bone and Mineral Research 14 (3): 351–61. doi:10.1359/jbmr.
1999.14.3.351. PMID 10027900.
| Image: Mesenchymal stem cell with prominent extensions and nuclei. |
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