• Mini Review, provided by StemCell, Inc., Ravenska Wagey, PhD, May 2008.

  • Mesenchymal Stem Cells: Promises and Reality, The Multiple Sclerosis Foundation, MS in focus
    Issue 11 - 2008.


Below are links to Adult Stem Cell online protocols, with emphasis on MSC protocols:

  • Invitrogen:  Protocols for isolation, characterization, differentiation, and expansion of adult
    stem cells from Invitrogen.

  • R&D Systems Protocols: Methods and products from R&D Systems.

  • Cold Spring Harbor Protocols:  New and classic research techniques for stem cell research,
    with a searchable database.

  • Current Protocols in Stem Cell Biology:  Online edition of the laboratory manual, updated
    monthly.  Subscription required.

  • Protocol Online:  Research protocols in a variety of life science fields, contributed by
    researchers worldwide and collected from the web, with discussion forums.

Note: To have your online protocols included in our list, please email your website to the Interest Group Editor.

Key References by Area:  


  • Bruder, S. P., Jaiswal, N., and Haynesworth, S. E. (1997) Growth kinetics, self-renewal, and
    the osteogenic potential of purified human mesenchymal stem cells during extensive
    subcultivation and following cryopreservation. J Cell Biochem 64, 278-294

  • Meirelles Lda, S., and Nardi, N. B. (2003) Murine marrow-derived mesenchymal stem cell:
    isolation, in vitro expansion, and characterization. Br J Haematol 123, 702-711

  • Phinney, D. G., Kopen, G., Isaacson, R. L., and Prockop, D. J. (1999) Plastic adherent stromal
    cells from the bone marrow of commonly used strains of inbred mice: variations in yield,
    growth, and differentiation. J Cell Biochem 72, 570-585


  • Deng, W., Obrocka, M., Fischer, I., and Prockop, D. J. (2001) In vitro differentiation of human
    marrow stromal cells into early progenitors of neural cells by conditions that increase
    intracellular cyclic AMP. Biochem Biophys Res Commun 282, 148-152

  • Mackay, A. M., Beck, S. C., Murphy, J. M., Barry, F. P., Chichester, C. O., and Pittenger, M. F.
    (1998) Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow.
    Tissue Eng 4, 415-428

  • Moscoso, I., Centeno, A., Lopez, E., Rodriguez-Barbosa, J. I., Santamarina, I., Filgueira, P.,
    Sanchez, M. J., Dominguez-Perles, R., Penuelas-Rivas, G., and Domenech, N. (2005)
    Differentiation "in vitro" of primary and immortalized porcine mesenchymal stem cells into
    cardiomyocytes for cell transplantation. Transplant Proc 37, 481-482


  • Anjos-Afonso, F., Siapati, E. K., and Bonnet, D. (2004) In vivo contribution of murine
    mesenchymal stem cells into multiple cell-types under minimal damage conditions. J Cell Sci
    117, 5655-5664

  • Djouad, F., Plence, P., Bony, C., Tropel, P., Apparailly, F., Sany, J., Noel, D., and Jorgensen,
    C. (2003) Immunosuppressive effect of mesenchymal stem cells favors tumor growth in
    allogeneic animals. Blood 102, 3837-3844

  • Gojo, S., Gojo, N., Takeda, Y., Mori, T., Abe, H., Kyo, S., Hata, J., and Umezawa, A. (2003) In
    vivo cardiovasculogenesis by direct injection of isolated adult mesenchymal stem cells. Exp
    Cell Res 288, 51-59

  • Kinnaird, T., Stabile, E., Burnett, M. S., Shou, M., Lee, C. W., Barr, S., Fuchs, S., and Epstein,
    S. E. (2004) Local delivery of marrow-derived stromal cells augments collateral perfusion
    through paracrine mechanisms. Circulation 109, 1543-1549

  • Olivares, E. L., Ribeiro, V. P., Werneck de Castro, J. P., Ribeiro, K. C., Mattos, E. C.,
    Goldenberg, R. C., Mill, J. G., Dohmann, H. F., dos Santos, R. R., de Carvalho, A. C., and
    Masuda, M. O. (2004) Bone marrow stromal cells improve cardiac performance in healed
    infarcted rat hearts. Am J Physiol Heart Circ Physiol 287, H464-470

  • Orlic, D., Kajstura, J., Chimenti, S., Jakoniuk, I., Anderson, S. M., Li, B., Pickel, J., McKay, R.,
    Nadal-Ginard, B., Bodine, D. M., Leri, A., and Anversa, P. (2001) Bone marrow cells
    regenerate infarcted myocardium. Nature 410, 701-705

  • Singh, S. K., Hawkins, C., Clarke, I. D., Squire, J. A., Bayani, J., Hide, T., Henkelman, R. M.,
    Cusimano, M. D., and Dirks, P. B. (2004) Identification of human brain tumour initiating cells.
    Nature 432, 396-401


  • De Ugarte, D. A., Morizono, K., Elbarbary, A., Alfonso, Z., Zuk, P. A., Zhu, M., Dragoo, J. L.,
    Ashjian, P., Thomas, B., Benhaim, P., Chen, I., Fraser, J., and Hedrick, M. H. (2003)
    Comparison of multi-lineage cells from human adipose tissue and bone marrow. Cells Tissues
    Organs 174, 101-109

  • Houghton, J., Stoicov, C., Nomura, S., Rogers, A. B., Carlson, J., Li, H., Cai, X., Fox, J. G.,
    Goldenring, J. R., and Wang, T. C. (2004) Gastric cancer originating from bone marrow-
    derived cells. Science 306, 1568-1571

  • Krampera, M., Glennie, S., Dyson, J., Scott, D., Laylor, R., Simpson, E., and Dazzi, F. (2003)
    Bone marrow mesenchymal stem cells inhibit the response of naive and memory antigen-
    specific T cells to their cognate peptide. Blood 101, 3722-3729

  • Oh, J. Y., Kim, M. K., Shin, M. S., Lee, H. J., Ko, J. H., Wee, W. R., and Lee, J. H. (2008) The
    anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound
    healing following chemical injury. Stem Cells 26, 1047-1055

  • Pittenger, M. F., Mackay, A. M., Beck, S. C., Jaiswal, R. K., Douglas, R., Mosca, J. D.,
    Moorman, M. A., Simonetti, D. W., Craig, S., and Marshak, D. R. (1999) Multilineage potential
    of adult human mesenchymal stem cells. Science 284, 143-147

  • Wakitani, S., Saito, T., and Caplan, A. I. (1995) Myogenic cells derived from rat bone marrow
    mesenchymal stem cells exposed to 5-azacytidine. Muscle Nerve 18, 1417-1426
Mesenchymal Stem Cells - Interest Group
MSC Resources
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